This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Addgene will be closed November 22nd & 23rd for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 19th - 23rd. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCYC1pr-GNSI (LEU)
(Plasmid #111450)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111450 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS415
  • Backbone size w/o insert (bp) 6021
  • Total vector size (bp) 8692
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CYC1 Promoter
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    290
  • Entrez Gene
    CYC1 (a.k.a. YJR048W)

Gene/Insert 2

  • Gene/Insert name
    Nyv1 Cytosolic Domain (aa 6-230)
  • Alt name
    GNS cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    675
  • Mutation
    M109I (Naturally occurring SNP)
  • Tag / Fusion Protein
    • mGFP5 (N terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    Snc1 Transmembrane Domain (TMD)
  • Alt name
    GNS cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    60

Gene/Insert 4

  • Gene/Insert name
    SUC2 CDS
  • Alt name
    GSI cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1539
  • Mutation
    The first 21 residues are deleted, removing the localization signal sequence

Gene/Insert 5

  • Gene/Insert name
    SUC2 terminator
  • Alt name
    GSI cassette
  • Insert Size (bp)
    459

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pGNS416, encoding GFP-Nyv1-Snc1TMD (GNS cassette) was a gift from F. Reggiori {Reggiori et al., 2000 PMID 11071903}. pHB4, encoding GSI (in earlier work called GSS) was a gift from E. Conibear {Burston et al., 2009, Addgene #53462}.
  • Terms and Licenses

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/05/04/314286 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCYC1pr-GNSI (LEU) was a gift from Alexey Merz (Addgene plasmid # 111450)
  • For your References section:

    Plate-based assay of AP-3 traffic in budding yeast. Plemel RL, Odorizzi G, Merz AJ. bioRxiv 314286 10.1101/314286