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(Plasmid #111450)


Item Catalog # Description Quantity Price (USD)
Plasmid 111450 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6021
  • Total vector size (bp) 8692
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CYC1 Promoter
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Entrez Gene
    CYC1 (a.k.a. YJR048W)

Gene/Insert 2

  • Gene/Insert name
    Nyv1 Cytosolic Domain (aa 6-230)
  • Alt name
    GNS cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Mutation
    M109I (Naturally occurring SNP)
  • Tag / Fusion Protein
    • mGFP5 (N terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    Snc1 Transmembrane Domain (TMD)
  • Alt name
    GNS cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)

Gene/Insert 4

  • Gene/Insert name
    SUC2 CDS
  • Alt name
    GSI cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Mutation
    The first 21 residues are deleted, removing the localization signal sequence

Gene/Insert 5

  • Gene/Insert name
    SUC2 terminator
  • Alt name
    GSI cassette
  • Insert Size (bp)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pGNS416, encoding GFP-Nyv1-Snc1TMD (GNS cassette) was a gift from F. Reggiori {Reggiori et al., 2000 PMID 11071903}. pHB4, encoding GSI (in earlier work called GSS) was a gift from E. Conibear {Burston et al., 2009, Addgene #53462}.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCYC1pr-GNSI (LEU) was a gift from Alexey Merz (Addgene plasmid # 111450 ; ; RRID:Addgene_111450)
  • For your References section:

    Plate-based assay of AP-3 traffic in budding yeast. Plemel RL, Odorizzi G, Merz AJ. bioRxiv 314286 10.1101/314286