|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111463||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameVal-tRNA synthetase
/ Fusion Protein
- 6xHis (C terminal on backbone)
- Cloning method Gibson Cloning
Terms and Licenses
Gene comes from E.coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:B1-1 was a gift from Cheemeng Tan (Addgene plasmid # 111463 ; http://n2t.net/addgene:111463 ; RRID:Addgene_111463)
For your References section:Synthetic microbial consortia enable rapid assembly of pure translation machinery. Villarreal F, Contreras-Llano LE, Chavez M, Ding Y, Fan J, Pan T, Tan C. Nat Chem Biol. 2018 Jan;14(1):29-35. doi: 10.1038/nchembio.2514. Epub 2017 Nov 13. 10.1038/nchembio.2514 PubMed 29131146