pJK1
(Plasmid #111722)

• Purpose: to express human BLM protein in yeast cells
• Depositing Lab: Ian Hickson
• Publication: Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Backbone

• Vector backbone: pYES2
• Backbone manufacturer: ThermoFisher
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BLM
• Alt name: BS
• Alt name: RECQ2
• Species: H. sapiens (human)
• Mutation: a C-terminal hexahistidine tag was added, a yeast Kozak consensus sequence was included upstream of the coding sequence, and the first five codons of S. cerevisiae TOP2 gene replaced the initiation codon of the BLM cDNA.
• Entrez Gene: BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
• Promoter: GAL1
• Tag / Fusion Protein:
  • hexahistidine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry