pGEMT-PT2A-Tdtomato-GFP-iRFP670
(Plasmid #111814)

• Purpose: Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A
• Depositing Lab: Li Qian
• Publication: Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2.

Backbone

• Vector backbone: pGEMT-easy
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 6200
• Vector type: cloning intermediate

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Tdtomato
• Insert Size (bp): 1400

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: M13 F/T7
• 3′ sequencing primer: P2A-r

Gene/Insert 2

• Gene/Insert name: GFP
• Insert Size (bp): 700

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: P2A-f
• 3′ sequencing primer: T2A-r

Gene/Insert 3

• Gene/Insert name: iRFP670
• Insert Size (bp): 900

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T2A-f
• 3′ sequencing primer: M13R/SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.