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pb-NES-ZapCV5 (cpV143)
(Plasmid #112061)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112061 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab uses Omnimax cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-ZapCV5
  • Species
    Synthetic
  • Insert Size (bp)
    1737
  • Mutation
    ECFP gene is truncated 36 bp at 3' end. Venus gene is circurlarly permuted at residue 173. Zap5 zinc binding domain derived from S. cerevisiae Zap1 zinc finger domains 1 & 2 with C581H, C586H, C618H and C623H mutations .
  • Promoter CBA
  • Tag / Fusion Protein
    • Nuclear Export Signal (NES) derived from HIV-1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Quintara Bio: pCX-cMyc-5 (5'-GCGAGCCGCAGCCATTGCCTTTTA-3')
  • 3′ sequencing primer Quintara Bio: IRES-Rev (5'-CCTCACATTGCCAAAAGACG-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pb-NES-ZapCV5 (cpV143) was a gift from Amy Palmer (Addgene plasmid # 112061 ; http://n2t.net/addgene:112061 ; RRID:Addgene_112061)
  • For your References section:

    Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors. Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer AE, Jimenez R. Anal Chem. 2017 Jan 3;89(1):711-719. doi: 10.1021/acs.analchem.6b03235. Epub 2016 Dec 13. 10.1021/acs.analchem.6b03235 PubMed 27959493