Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11244||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2500
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameAdML-three MS2 hairpins
/ Fusion Protein
- 3 MS2 hairpins (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Reed lab clone #388.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AdML-M3 was a gift from Robin Reed (Addgene plasmid # 11244 ; http://n2t.net/addgene:11244 ; RRID:Addgene_11244)
For your References section:Purification and electron microscopic visualization of functional human spliceosomes. Zhou Z, Sim J, Griffith J, Reed R. Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7. 10.1073/pnas.182427099 PubMed 12215496
Map uploaded by the depositor.