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pG1-NMGR526(d22-69)
(Plasmid #1125)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1125 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pG1, 2 micron GPD promoter PGK terminator, TRP selection
  • Backbone size w/o insert (bp) 7400
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sup35 NM domain fused with GR
  • Alt name
    Rat glucocorticoid receptor
  • Alt name
    Yeast Sup35
  • Species
    R. norvegicus (rat), S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2193
  • Mutation
    nucleotides encoding NM domain amino acids 22-69 have been deleted
  • GenBank ID
    AY553990
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Entrez Gene
    Nr3c1 (a.k.a. GR, Gcr, Grl)
  • Tag / Fusion Protein
    • GR526 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    see comments
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG1-NMGR526(d22-69) was a gift from Susan Lindquist (Addgene plasmid # 1125 ; http://n2t.net/addgene:1125 ; RRID:Addgene_1125)
  • For your References section:

    Creating a protein-based element of inheritance. Li L, Lindquist S. Science 2000 Jan 28;287(5453):661-4. 10.1126/science.287.5453.661 PubMed 10650001