pSopBWT
(Plasmid
#112512)
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PurposePsopB driven sopB and sigE
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWSK29
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesopB/sigE operon
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Alt namesigDE
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SpeciesSalmonella enterica serovar Typhimurium
- Promoter PsopB
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSopBWT was a gift from Olivia Steele-Mortimer (Addgene plasmid # 112512 ; http://n2t.net/addgene:112512 ; RRID:Addgene_112512) -
For your References section:
Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane. Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O. Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13. 10.1111/j.1462-5822.2009.01356.x PubMed 19614667
Map uploaded by the depositor.
