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pCTD26-S>A2-9
(Plasmid #112825)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112825 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJMD2
  • Backbone size w/o insert (bp) 5345
  • Total vector size (bp) 5957
  • Vector type
    Yeast Expression
  • Selectable markers
    Gentamicin, LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPB1
  • Alt name
    RPO21
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5200
  • Mutation
    pSMF2 with all S to A mutations in only repeats 2-9.
  • Entrez Gene
    RPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)
  • Promoter Native promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xma1 (not destroyed)
  • 3′ cloning site Sac1 (not destroyed)
  • 5′ sequencing primer GATCGATGAGGAGTCACTGG
  • 3′ sequencing primer TTTACTAGCGCCGTTGGTTT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCTD26-S>A2-9 was a gift from Stephen Fuchs (Addgene plasmid # 112825 ; http://n2t.net/addgene:112825 ; RRID:Addgene_112825)
  • For your References section:

    Repeat-Specific Functions for the C-Terminal Domain of RNA Polymerase II in Budding Yeast. Babokhov M, Mosaheb MM, Baker RW, Fuchs SM. G3 (Bethesda). 2018 May 4;8(5):1593-1601. doi: 10.1534/g3.118.200086. 10.1534/g3.118.200086 PubMed 29523636