pcDNA3.1(+)/Puro/TagRFP-LL5b
(Plasmid
#112828)
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Purposetransient/stable overexpression
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1(+)/Puro
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLL5b
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SpeciesM. musculus (mouse)
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Entrez GenePhldb2 (a.k.a. AV253284, C820004H04Rik, LL, LL5b, LL5beta)
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Tag
/ Fusion Protein
- TagRFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LL5b contains a P815S difference compared to NM_153412.4 that does not impact function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)/Puro/TagRFP-LL5b was a gift from Tomasz Prószyński & Joshua Sanes (Addgene plasmid # 112828 ; http://n2t.net/addgene:112828 ; RRID:Addgene_112828) -
For your References section:
Amotl2 interacts with LL5beta, localizes to podosomes and regulates postsynaptic differentiation in muscle. Proszynski TJ, Sanes JR. J Cell Sci. 2013 May 15;126(Pt 10):2225-35. doi: 10.1242/jcs.121327. Epub 2013 Mar 22. 10.1242/jcs.121327 PubMed 23525008
