Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #112911)


Item Catalog # Description Quantity Price (USD)
Plasmid 112911 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    protein 4.9
  • Alt name
    48 kDa form of human dematin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAGKVWAD102
  • GenBank ID
  • Entrez Gene
    DMTN (a.k.a. DMT, EPB49)
  • Promoter T7
  • Tags / Fusion Proteins
    • Glutathione-S-Transferase (N terminal on backbone)
    • 6-his tag (C terminal on insert)
    • precission protease site between GST and dematin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_rev_primer
  • 3′ sequencing primer pGEX_3_primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Athar Chishti, Tufts University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rd-his was a gift from C. James McKnight (Addgene plasmid # 112911 ; ; RRID:Addgene_112911)
  • For your References section:

    The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). Chen L, Brown JW, Mok YF, Hatters DM, McKnight CJ. J Biol Chem. 2013 Mar 22;288(12):8313-20. doi: 10.1074/jbc.M112.438861. Epub 2013 Jan 25. 10.1074/jbc.M112.438861 PubMed 23355471