KVS1-HA
(Plasmid
#113061)
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PurposeExpresses worm KVS-1 in mammalian cells
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Depositing Lab
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Depositing OrganizationRutgers, The State University of New Jersey
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 113061-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 113061-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCI-neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK+ voltage sensitive channel subunit 1
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SpeciesC. elegans (nematode)
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Entrez Genekvs-1 (a.k.a. CELE_C53C9.3)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
DNA (Catalog # 113061-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 113061-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KVS1-HA was a gift from Federico Sesti (Addgene plasmid # 113061 ; http://n2t.net/addgene:113061 ; RRID:Addgene_113061) -
For your References section:
MPS-1 is a K+ channel beta-subunit and a serine/threonine kinase. Cai SQ, Hernandez L, Wang Y, Park KH, Sesti F. Nat Neurosci. 2005 Nov;8(11):1503-9. doi: 10.1038/nn1557. Epub 2005 Oct 16. 10.1038/nn1557 PubMed 16222231
