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pEBLindv2
(Plasmid #113108)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113108 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBbE8K
  • Modifications to backbone
    First insert: J23106-BBa_B0034-EL222-Bba_B0015; followed by second insert: BindF1-Bba_B0034-RFP-Bba_B0015
  • Vector type
    Bacterial Expression ; Cell-free

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EL222
  • Species
    E. coli
  • Promoter J23106
  • Tag / Fusion Protein
    • RFP (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBLindv2 was a gift from Chueh Loo Poh (Addgene plasmid # 113108)
  • For your References section:

    Cell-Free Optogenetic Gene Expression System. Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL. ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 10.1021/acssynbio.7b00422. Epub 2018 Apr 4. 10.1021/acssynbio.7b00422 PubMed 29596741