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pRL-TK let7 A
(Plasmid #11324)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 11324 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4045
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    2 binding sites for let-7a miRNA
  • Mutation
    Mutation A from figure 4A of paper.
  • Tag / Fusion Protein
    • Rr-luc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comment (unknown if destroyed)
  • 3′ cloning site See comment (unknown if destroyed)
  • 5′ sequencing primer Don't know
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL-TK let7 A was a gift from Phil Sharp (Addgene plasmid # 11324 ; ; RRID:Addgene_11324)
  • For your References section:

    Specificity of microRNA target selection in translational repression. Doench JG, Sharp PA. Genes Dev. 2004 Mar 1. 18(5):504-11. 10.1101/gad.1184404 PubMed 15014042