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pCRISPR
(Plasmid #113252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113252 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3240
  • Vector type
    Integration substrate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E. coli CRISPR array
  • Species
    S. pyogenes
  • Insert Size (bp)
    964
  • GenBank ID
    LN832404.1
  • Promoter NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AflIII (not destroyed)
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR was a gift from Jennifer Doudna (Addgene plasmid # 113252 ; http://n2t.net/addgene:113252 ; RRID:Addgene_113252)
  • For your References section:

    Protecting genome integrity during CRISPR immune adaptation. Wright AV, Doudna JA. Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 10.1038/nsmb.3289. Epub 2016 Sep 5. 10.1038/nsmb.3289 PubMed 27595346