pJY3948
(Plasmid #113338)

• Purpose: 6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
• Depositing Lab: Suzanne Sandmeyer
• Publication: Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.1002/bit.26733.

Backbone

• Vector backbone: pJY3872
• Total vector size (bp): 7894
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 2-pyrone synthase, codon optimized for Y. lipolytica
• Alt name: 2-PS
• Species: Gerbera hybrida
• Insert Size (bp): 1239
• Mutation: Please see depositor comments
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Plasmid contains a G296C mutation in 2-pyrone synthase. This mutation is not known to affect plasmid function.