|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113426||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3399
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human); S. pyogenes and Bacteriophage PBS2
Insert Size (bp)5043
Entrez GeneAPOBEC3A (a.k.a. A3A, ARP3, PHRBN, bK150C2.1)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH poly reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-hA3A-BE3-C101S was a gift from Jia Chen (Addgene plasmid # 113426 ; http://n2t.net/addgene:113426 ; RRID:Addgene_113426)
For your References section:Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol., 2018 10.1038/nbt.4198