pRST7-ER beta (long)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11356||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameER beta long
SpeciesH. sapiens (human)
Insert Size (bp)1590
Entrez GeneESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRST7-ER beta (long) was a gift from Donald McDonnell (Addgene plasmid # 11356 ; http://n2t.net/addgene:11356 ; RRID:Addgene_11356)
For your References section:The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. Hall JM, McDonnell DP. Endocrinology. 1999 Dec . 140(12):5566-78. 10.1210/endo.140.12.7179 PubMed 10579320