PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113644||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepACYC184. p15A
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Gentamycin, 10 μg/mL
Copy numberLow Copy
Gene/Insert nameMjYRS and tRNA gene
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRF0G-Tyr was a gift from Jeffrey Barrick (Addgene plasmid # 113644 ; http://n2t.net/addgene:113644 ; RRID:Addgene_113644)
For your References section:Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli. Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE. ACS Synth Biol. 2016 Sep 20. 10.1021/acssynbio.6b00192 PubMed 27648665