PurposeCRISPR donor plasmid to tag human transcription factor SPDEF with GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113828||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSPDEF homology arms flanking EGFP-IRES-Neo cassette
SpeciesH. sapiens (human)
Entrez GeneSPDEF (a.k.a. PDEF, bA375E1.3)
- Cloning method Gibson Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Cloning source: MCF-7.
Use with plasmid pSPDEF.1.0-gDNA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSPDEF-donor was a gift from Kevin White (Addgene plasmid # 113828 ; http://n2t.net/addgene:113828 ; RRID:Addgene_113828)