|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11390||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4733
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameADP Ribosylation Factor 1
SpeciesH. sapiens (human)
Insert Size (bp)545
Entrez GeneARF1 (a.k.a. PVNH8)
/ Fusion Protein
- YFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer GGTGGGAGGTCTATATAAGCAGAGCTGGTT
- 3′ sequencing primer CCCCGGTGAACAGCTCCTCGCCCTTGCTCA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byhARF1 was obtained from the UMR cDNA Resource center (www.cDNA.org).
Terms and Licenses
- Not Available to Industry
Encodes a YFP fusion to the C-terminus of ARF1. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP variant.
Please note that the XbaI site depicted in the map is not actually present in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pARF1-YFP was a gift from Joel Swanson (Addgene plasmid # 11390 ; http://n2t.net/addgene:11390 ; RRID:Addgene_11390)
For your References section:A Phosphatidylinositol-3-Kinase-Dependent Signal Transition Regulates ARF1 and ARF6 during Fcgamma Receptor-Mediated Phagocytosis. Beemiller P, Hoppe AD, Swanson JA. PLoS Biol. 2006 May 9. 4(6):e162. 10.1371/journal.pbio.0040162 PubMed 16669702