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pCEP4-HA-Flag-natT1R2 FS1
(Plasmid #113955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113955 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 10393
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T1R2
  • Alt name
    natT1R2
  • Alt name
    hT1R2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2552
  • Mutation
    delete N-terminal 21 residues, one base pair deletion at position 837
  • GenBank ID
    NM_152232.2
  • Entrez Gene
    TAS1R2 (a.k.a. GPR71, T1R2, TR2)
  • Promoter cmv
  • Tags / Fusion Proteins
    • Signal/leader sequence from influenza hemagglutinin (HA) (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-HA-Flag-natT1R2 FS1 was a gift from Erik Procko (Addgene plasmid # 113955 ; http://n2t.net/addgene:113955 ; RRID:Addgene_113955)
  • For your References section:

    Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits. Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E. J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173. 10.1074/jbc.RA118.006173 PubMed 30723160