pENTR1a-tagRFP-KDEL
(Plasmid #114177)

• Purpose: Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER
• Depositing Lab: Nicolas Demaurex
• Publication: Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.

Backbone

• Vector backbone: pENTR1a
• Backbone manufacturer: Invitrogen/Thermo
• Backbone size w/o insert (bp): 2717
• Vector type: Gateway entry clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tagRFP-KDEL
• Alt name: ER-RFP
• Species: Synthetic
• Insert Size (bp): 831
• Tags / Fusion Proteins:
  • signal peptide (N terminal on insert)
  • KDEL retention signal (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry