Depositor Sequences: None.
Addgene Sequences: Partial (2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11421||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5386
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1032
GenBank IDAAD36840 NP_229574 TM1777
/ Fusion Proteins
- 6x His (N terminal on backbone)
- TEV protease site (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pSKB3.TM1777 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSKB3.TM1777 was a gift from Sung-Hou Kim (Addgene plasmid # 11421)
For your References section:Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain. Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH. Biochemistry. 2003 Nov 25. 42(46):13429-37. 10.1021/bi035118h PubMed 14621988