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(Plasmid #114211)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114211 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    pBluescript SKII+
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 11800
  • Modifications to backbone
    Restriction sites added and rearranged in the vector MCS.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    aP2 promoter + pOB4 + eGFPtopaz
  • Alt name
    Fabp4 promoter + splicing intron + Topaz
  • Species
    M. musculus (mouse), Synthetic; Aequorea victoria
  • GenBank ID
  • Entrez Gene
    Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
  • Promoter aP2/Fabp4
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc65 1 (destroyed during cloning)
  • 3′ cloning site Acc65 1 (destroyed during cloning)
  • 5′ sequencing primer T3 or M13R
  • 3′ sequencing primer T7 or M13F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    7.7 kb aP2 promoter was received from Dr. Charles Vinson at NCI/NIH. Topaz GFP was purchased from Packard (originally from Aurora).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct was used to create transgenic mice and results of analyses of tissues from these animals were published in meeting abstracts appearing in 2004 and 2008 issues of the Journal of Bone & Mineral Research.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aP2-GFPtpz was a gift from David Rowe (Addgene plasmid # 114211 ; ; RRID:Addgene_114211)
  • For your References section:

    Mechanical input restrains PPARgamma2 expression and action to preserve mesenchymal stem cell multipotentiality. Case N, Thomas J, Xie Z, Sen B, Styner M, Rowe D, Rubin J. Bone. 2013 Jan;52(1):454-64. doi: 10.1016/j.bone.2012.08.122. Epub 2012 Aug 23. 10.1016/j.bone.2012.08.122 PubMed 22951341