aP2-GFPtpz
(Plasmid #114211)

• Purpose: aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage
• Depositing Lab: David Rowe
• Depositing Organization: University of Connecticut Health Center
  Located in United States of America
• Publication: Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.1016/j.bone.2012.08.122. Epub 2012 Aug 23.

Backbone

• Vector backbone: pBluescript SKII+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 11800
• Modifications to backbone: Restriction sites added and rearranged in the vector MCS.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aP2 promoter + pOB4 + eGFPtopaz
• Alt name: Fabp4 promoter + splicing intron + Topaz
• Species: M. musculus (mouse), Synthetic; Aequorea victoria
• GenBank ID:
• Entrez Gene: Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
• Promoter: aP2/Fabp4
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Acc65 1 (destroyed during cloning)
• 3′ cloning site: Acc65 1 (destroyed during cloning)
• 5′ sequencing primer: T3 or M13R
• 3′ sequencing primer: T7 or M13F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 7.7 kb aP2 promoter was received from Dr. Charles Vinson at NCI/NIH. Topaz GFP was purchased from Packard (originally from Aurora).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct was used to create transgenic mice and results of analyses of tissues from these animals were published in meeting abstracts appearing in 2004 and 2008 issues of the Journal of Bone & Mineral Research.

DNA (Catalog # 114211-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 114211-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.