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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)
(Plasmid #114212)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114212 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR (lentivirus)
  • Total vector size (bp) 10089
  • Modifications to backbone
    8 miR-128 target sites and 8 miR-221 target sites included in 3' UTR
  • Vector type
    Mammalian Expression, Mouse Targeting, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    EGFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    717
  • Mutation
    None
  • Promoter human synapsin (hSyn)
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-hSyn-EGFP-8X2C (LV GABA mAGNET) was a gift from Xue Han (Addgene plasmid # 114212 ; http://n2t.net/addgene:114212 ; RRID:Addgene_114212)
  • For your References section:

    A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X. Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. 10.1016/j.celrep.2018.06.049 PubMed 29996091