Depositor Sequences: None.
Addgene Sequences: None. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11432||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5386
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameribosome binding protein
Insert Size (bp)348
GenBank IDAAB96323 NP_109844 MPN156
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pSKB3.MPN156 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSKB3.MPN156 was a gift from Sung-Hou Kim (Addgene plasmid # 11432)
For your References section:Solution structure of a putative ribosome binding protein from Mycoplasma pneumoniae and comparison to a distant homolog. Rubin SM, Pelton JG, Yokota H, Kim R, Wemmer DE. J Struct Funct Genomics. 2003 . 4(4):235-43. 10.1023/B:JSFG.0000016127.57320.82 PubMed 15185964