Purpose(Empty Backbone) Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114321||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCWX-R4DESTR2-PGK-Tk:Sh was a gift from Patrick Salmon (Addgene plasmid # 114321 ; http://n2t.net/addgene:114321 ; RRID:Addgene_114321)