Purposeanchor iLID domain to plasma membrane with slower diffusion
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114417||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameiLID domain
- Promoter CMV
/ Fusion Protein
- eGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:iLID-eGFP-3xC2 was a gift from Dorus Gadella (Addgene plasmid # 114417 ; http://n2t.net/addgene:114417 ; RRID:Addgene_114417)
For your References section:Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Van Geel O, Hartsuiker R, Gadella TWJ. Small GTPases. 2018 Sep 5:1-10. doi: 10.1080/21541248.2018.1507411. 10.1080/21541248.2018.1507411 PubMed 30182785