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pHMMa.PH0999
(Plasmid #11453)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11453 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pyrazinamidase
  • Species
    P. horikoshii
  • Insert Size (bp)
    540
  • GenBank ID
    BAA30096 PH0999 NP_142913
  • Entrez Gene
    PH0999

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that the plasmid pHMMa.PH0999 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMMa.PH0999 was a gift from Sung-Hou Kim (Addgene plasmid # 11453)
  • For your References section:

    Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH. Biochemistry. 2001 Nov 27. 40(47):14166-72. 10.1021/bi0115479 PubMed 11714269