pcmvtag2Bmciap2 mut
(Plasmid #11464)

• Depositing Lab: Jon Ashwell
• Publication: Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Backbone

• Vector backbone: pcmvtag2B
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mciap2mut
• Alt name: Birc3
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: H570A
• Entrez Gene: Birc3 (a.k.a. A, AW107670, Api1, Api2, Birc2, C-IAP2, HI, HIAP2, I, IAP1, IAP2, MI, MIAP1, MIAP2, MIH, MIHB, MIHC, RNF, RNF49, cI, cIA, cIAP-1, cIAP-2, cIAP1, cIAP2)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are several additional mutations in this construct - R398W, D419E, R445W and I591V. The depositing lab is unaware of any effects these mutations may have on plasmid function. Compared to the wild type, the H570A mutation alone renders the E3 enzymatically inactive.