Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #115171)


Item Catalog # Description Quantity Price (USD)
Plasmid 115171 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Semaphorin 4A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    First 32 amino acids of Sema4A replaced with membrane targeting sequence
  • GenBank ID
  • Entrez Gene
    Sema4a (a.k.a. AI132332, SemB, Semab)
  • Promoter CMV
  • Tags / Fusion Proteins
    • membrane targeting sequence (N terminal on backbone)
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcttatggccatggaggcccgaACTGGTGGTCAGGGGCCC
  • 3′ sequencing primer tcgagagatctcggtcgaccgTCAAGCCACTTCGGCGCC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-myc-Sema4A was a gift from Suzanne Paradis (Addgene plasmid # 115171 ; ; RRID:Addgene_115171)
  • For your References section:

    Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. McDermott JE, Goldblatt D, Paradis S. Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 10.1016/j.mcn.2018.06.008. 10.1016/j.mcn.2018.06.008 PubMed 29981480