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pAcUW31-M10.5/human b2m
(Plasmid #11521)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11521 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcUW31
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 8532
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M10.5
  • Alt name
    M10.5 ectodomain
  • Alt name
    major histocompatibility complex class Ib M10.5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    948
  • GenBank ID
    AY211081
  • Entrez Gene
    H2-M10.4 (a.k.a. 15H)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer 5' CCg gAT CTg ATC ATg gAg ATA A 3'
  • 3′ sequencing primer 5' TCg CTC TAA CAT ACC ACC CT 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    C. Dulac
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcUW31-M10.5/human b2m was a gift from Pamela Bjorkman (Addgene plasmid # 11521 ; http://n2t.net/addgene:11521 ; RRID:Addgene_11521)
  • For your References section:

    Structure of a pheromone receptor-associated MHC molecule with an open and empty groove. Olson R, Huey-Tubman KE, Dulac C, Bjorkman PJ. PLoS Biol. 2005 Aug . 3(8):e257. 10.1371/journal.pbio.0030257 PubMed 16089503