|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11249||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecathepsin B
SpeciesH. sapiens (human)
Entrez GeneCTSB (a.k.a. APPS, CPSB, RECEUP)
/ Fusion Protein
- C9 (GTETSQVAPA) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hCathepsin B was a gift from Hyeryun Choe (Addgene plasmid # 11249 ; http://n2t.net/addgene:11249 ; RRID:Addgene_11249)
For your References section:SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells. Huang IC, Bosch BJ, Li F, Li W, Lee KH, Ghiran S, Vasilieva N, Dermody TS, Harrison SC, Dormitzer PR, Farzan M, Rottier PJ, Choe H. J Biol Chem. 2005 Dec 8. ():. 10.1074/jbc.M508381200 PubMed 16339146