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hCathepsin L
(Plasmid #11250)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11250 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *
Cloning Grade DNA 11250-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cathepsin L
  • Species
    H. sapiens (human)
  • Entrez Gene
    CTSL (a.k.a. CATL, CTSL1, MEP)
  • Tag / Fusion Protein
    • C9 (GTETSQVAPA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map for more information.

Information for Cloning Grade DNA (Catalog # 11250-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCathepsin L was a gift from Hyeryun Choe (Addgene plasmid # 11250 ; http://n2t.net/addgene:11250 ; RRID:Addgene_11250)
  • For your References section:

    SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells. Huang IC, Bosch BJ, Li F, Li W, Lee KH, Ghiran S, Vasilieva N, Dermody TS, Harrison SC, Dormitzer PR, Farzan M, Rottier PJ, Choe H. J Biol Chem. 2005 Dec 8. ():. 10.1074/jbc.M508381200 PubMed 16339146