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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
(Plasmid #115216)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115216 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    SUMO bacterial expression vector
  • Backbone size w/o insert (bp) 6087
  • Total vector size (bp) 9447
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pin2Cas13
  • Alt name
    Cas13a from Prevotella intermedia
  • Species
    Prevotella intermedia
  • Insert Size (bp)
    3360
  • Tag / Fusion Protein
    • SUMO

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI was a gift from Feng Zhang (Addgene plasmid # 115216 ; http://n2t.net/addgene:115216 ; RRID:Addgene_115216)
  • For your References section:

    Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. 10.1126/science.aaq0179 PubMed 29449508