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pAAV-UbC-mEmerald Rab7a
(Plasmid #115238)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115238 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-SIBR-anti_I
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 6627
  • Modifications to backbone
    The anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab7a fused to mEmerald
  • Alt name
    NM_004637.5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1416
  • Mutation
    7 Amino Acids (21 Nucleotides) between mEmerald and Rab7a
  • GenBank ID
    NM_004637.5
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter Human UbC
  • Tags / Fusion Proteins
    • mEmerald (N terminal on insert)
    • mEmerald (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer pCAG_F_primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ADDGENE plasmid #54244
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-mEmerald Rab7a was a gift from Pantelis Tsoulfas (Addgene plasmid # 115238 ; http://n2t.net/addgene:115238 ; RRID:Addgene_115238)