Depositor Sequences: None.
Addgene Sequences: Partial (1)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11527||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6194
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)705
GenBank IDAAD36799 TM1734 NP_229532
Entrez GeneTM1734 (a.k.a. TM1734)
/ Fusion Protein
- HisTag, TEV cleavable (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pLIC.B3.TM1734 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIC.B3.TM1734 was a gift from Sung-Hou Kim (Addgene plasmid # 11527)
For your References section:Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters. Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH. J Biol Chem. 2005 Apr 22. 280(16):15960-6. 10.1074/jbc.M414117200 PubMed 15716271
Generated by Addgene from plasmid data.