|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11531||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7313
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)993
GenBank IDNP_269282 SPy1128
/ Fusion Protein
- HisTag-MBP, TEV cleavable (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer CGTCAGACTGTCGATGAAGCCC
- 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pLIC.B4.SPy1128 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIC.B4.SPy1128 was a gift from Sung-Hou Kim (Addgene plasmid # 11531 ; http://n2t.net/addgene:11531 ; RRID:Addgene_11531)
For your References section:Crystal structure of a phosphotransacetylase from Streptococcus pyogenes. Xu QS, Shin DH, Pufan R, Yokota H, Kim R, Kim SH. Proteins. 2004 May 1. 55(2):479-81. 10.1002/prot.20039 PubMed 15048838