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pLIC.B4.SPy1128
(Plasmid #11531)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11531 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphotransacetylase
  • Species
    S. pyogenes
  • Insert Size (bp)
    993
  • GenBank ID
    NP_269282 SPy1128
  • Tag / Fusion Protein
    • HisTag-MBP, TEV cleavable (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer CGTCAGACTGTCGATGAAGCCC
  • 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that the plasmid pLIC.B4.SPy1128 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC.B4.SPy1128 was a gift from Sung-Hou Kim (Addgene plasmid # 11531)
  • For your References section:

    Crystal structure of a phosphotransacetylase from Streptococcus pyogenes. Xu QS, Shin DH, Pufan R, Yokota H, Kim R, Kim SH. Proteins. 2004 May 1. 55(2):479-81. 10.1002/prot.20039 PubMed 15048838