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pFL-SV40-Rac1 5'-UTR
(Plasmid #115354)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115354 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 115354-D50 50 μg of DNA in Tris buffer $495
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DNA 115354-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFL-SV40
  • Total vector size (bp) 4430
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rac1 5'-UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    241
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFL-SV40-Rac1 5'-UTR was a gift from Ming-Chih Lai (Addgene plasmid # 115354 ; http://n2t.net/addgene:115354 ; RRID:Addgene_115354)

  • For your References section:

    DDX3 Participates in Translational Control of Inflammation Induced by Infections and Injuries. Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC. Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 10.1128/MCB.00285-18. Print 2019 Jan 1. 10.1128/MCB.00285-18 PubMed 30373933
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