GFP-PIWI
(Plasmid
#11591)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonecustom-made pDEST:gfp vector
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAgo2
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Alt nameEIF2C2
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Mutationcontain only aa482-869
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Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GACCGCCGCCGGGATCACT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-PIWI was a gift from Edward Chan (Addgene plasmid # 11591 ; http://n2t.net/addgene:11591 ; RRID:Addgene_11591) -
For your References section:
Disruption of GW bodies impairs mammalian RNA interference. Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel JC, Fritzler MJ, Chan EK. Nat Cell Biol. 2005 Dec . 7(12):1267-74. 10.1038/ncb1334 PubMed 16284622