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pGEX2T merlin 1-595
(Plasmid #11629)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11629 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    merlin
  • Alt name
    NF2
  • Alt name
    isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1785
  • Entrez Gene
    NF2 (a.k.a. ACN, BANF, SCH)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

The merlin insert in this plasmid contains A190V and G302E mutations compared to the closest match human Merlin reference sequence (NP_000259.1). The depositing laboratory states that these mutations should not affect merlin function, but cautions that it may be necessary to test merlin function if performing structure function experiments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX2T merlin 1-595 was a gift from Vijaya Ramesh (Addgene plasmid # 11629 ; http://n2t.net/addgene:11629 ; RRID:Addgene_11629)
  • For your References section:

    Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF. Gonzalez-Agosti C, Wiederhold T, Herndon ME, Gusella J, Ramesh V. J Biol Chem. 1999 Nov 26. 274(48):34438-42. 10.1074/jbc.274.48.34438 PubMed 10567424