pFGL1260R
(Plasmid #116901)

• Purpose: mCherry:TubA (tubulin marker)
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL1259R
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 11085
• Total vector size (bp): 14657
• Modifications to backbone: mCherry:TubA
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Sulfonylurea (Chlorimuron ethyl)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TubA promoter
• Species: Magnaporthe oryzae
• Insert Size (bp): 1057

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: GCACTCGAGGCCGCCGGTGTAATTCATGGTGACT
• 3′ sequencing primer: CTCGGTACCCATTGTGGATTCTAGGCACTTTTCTCAG

Gene/Insert 2

• Gene/Insert name: TubA (ORF+3'UTR)
• Species: Magnaporthe oryzae
• Insert Size (bp): 2556

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: TCCGGATCCAAAGGCGAGGTAGGTGATGCTT
• 3′ sequencing primer: GCGCTCTAGAGGGTGAAACCAAGTATGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry