pFGL1344
(Plasmid #116902)

• Purpose: Alp6:Bar:mCherry (SPB/MTOC marker)
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL1269R
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 8096
• Total vector size (bp): 10046
• Modifications to backbone: Alp6:Bar:mCherry
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Alp6 (partial ORF)
• Species: Magnaporthe oryzae
• Insert Size (bp): 1015

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (not destroyed)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: CTCGAATTCGCAATATGCAAGCCCAGAAGTGC
• 3′ sequencing primer: AACGGTACCGTCTCCAGCACGCTCGCCCCTG

Gene/Insert 2

• Gene/Insert name: Alp6 downstream region
• Insert Size (bp): 982

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: TGCTCTAGATAAGTAGTTTGAGAAGGAATGACGC
• 3′ sequencing primer: ATGAAGCTTGGGTCGATGAGTGGTTGGGTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry