pFGL1258
(Plasmid
#116898)
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PurposeTetOFF:GFP:CenpA (centromere marker)
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Depositing Lab
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Depositing OrganizationTemasek Life Sciences Laboratory
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 116898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 116898-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 116898-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFGL125_TetGFP(Hyg)
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Backbone manufacturerNaweed Lab
- Backbone size w/o insert (bp) 11398
- Total vector size (bp) 13113
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Modifications to backboneCenpA was introduced.
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Vector typeFungal expression (in Magnaporthe oryzae)
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCenpA promoter region
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SpeciesMagnaporthe oryzae
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Insert Size (bp)809
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR I (not destroyed)
- 3′ cloning site EcoR I (not destroyed)
- 5′ sequencing primer ggtGAATTCagtcgcaggtacatctcatta
- 3′ sequencing primer gtgGAATTCtttatgtcggtttctatatggtttc
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCenpA
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SpeciesMagnaporthe oryzae
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Insert Size (bp)930
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer ATAggatccCCACCACAAAAAGTAAAGAAGG
- 3′ sequencing primer gttTCTAGAaaagcagtccccagagtaactt
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 116898-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 116898-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFGL1258 was a gift from Naweed Naqvi (Addgene plasmid # 116898 ; http://n2t.net/addgene:116898 ; RRID:Addgene_116898) -
For your References section:
Cellular Dynamics and Genomic Identity of Centromeres in Cereal Blast Fungus. Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI. MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19. 10.1128/mBio.01581-19 PubMed 31363034
