pFGL1169R
(Plasmid #116899)

• Purpose: CenpC:mCherry (centromere marker)
• Depositing Lab: Naweed Naqvi
• Publication: Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 10.1128/mBio.01581-19.

Backbone

• Vector backbone: pFGL822
• Backbone manufacturer: Naweed Lab
• Backbone size w/o insert (bp): 7779
• Total vector size (bp): 9989
• Modifications to backbone: insertion with (truncated) CenpC-mCherry
• Vector type: Fungal expression (in Magnaporthe oryzae)
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cenp C (partial)
• Species: Magnaporthe oryzae
• Insert Size (bp): 562
• Mutation: truncated, no stop codon

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (not destroyed)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: CCGGAATTCGAGGACGAAGAACC
• 3′ sequencing primer: GATGGTACCGCTGCTTTCAGTCATTTCGTCC

Gene/Insert 2

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 720
• Mutation: without start codon

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: CTCGGTACCGTGAGCAAGGGCGAGGAGGATAA
• 3′ sequencing primer: CGCGGATCCTTACTTGTACAGCTCGTCCATGC

Gene/Insert 3

• Gene/Insert name: CenpC downstream region
• Species: Magnaporthe oryzea
• Insert Size (bp): 981

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: Pst I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: CTGCTGCAGTAATTCATCGCGGTGGGTTGGTC
• 3′ sequencing primer: TGTAAGCTTCTGGCCCTCCCTCATTAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry