PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||116984||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameAzospirillum lipoferum 4B RT-Cas1-Cas2
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC was a gift from Randall Platt (Addgene plasmid # 116984 ; http://n2t.net/addgene:116984 ; RRID:Addgene_116984)
For your References section:Transcriptional recording by CRISPR spacer acquisition from RNA. Schmidt F, Cherepkova MY, Platt RJ. Nature. 2018 Oct;562(7727):380-385. doi: 10.1038/s41586-018-0569-1. Epub 2018 Oct 3. 10.1038/s41586-018-0569-1 PubMed 30283135