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pCMV5B-FLAG-SARA
(Plasmid #11738)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11738 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 11738-D50 50 μg of DNA in Tris buffer $495 *
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DNA 11738-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • GenBank ID
    AF104304
  • Entrez Gene
    ZFYVE9 (a.k.a. MADHIP, NSP, PPP1R173, SARA, SMADIP)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-FLAG-SARA was a gift from Jeff Wrana (Addgene plasmid # 11738 ; http://n2t.net/addgene:11738 ; RRID:Addgene_11738)

  • For your References section:

    SARA, a FYVE domain protein that recruits Smad2 to the TGFbeta receptor. Tsukazaki T, Chiang TA, Davison AF, Attisano L, Wrana JL. Cell. 1998 Dec 11. 95(6):779-91. 10.1016/S0092-8674(00)81701-8 PubMed 9865696
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