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pAX2
(Plasmid #117398)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117398 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18R6K
  • Modifications to backbone
    A scaffold with interspersed restriction sites containing genes encoding chloramphenicol and gentamicin resistance and GFP was inserted into the backbone. In addition, the temperature-sensitive origin of replication ori101/repA101ts was inserted between the blunt restriction sites PsiI and SnaBI, destroying the R6K origin of replication. A synthetic kill switch consisting of a gene encoding the Tet repressor and a tet-controlled synthetic operon containing the genes hokB, ghoT, and tisB was also inserted into the backbone.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Also carries chloramphenicol and gentamicin selectable markers.
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAX2 was a gift from Karen Guillemin (Addgene plasmid # 117398 ; http://n2t.net/addgene:117398 ; RRID:Addgene_117398)
  • For your References section:

    Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages. Wiles TJ, Wall ES, Schlomann BH, Hay EA, Parthasarathy R, Guillemin K. MBio. 2018 Oct 9;9(5). pii: mBio.01877-18. doi: 10.1128/mBio.01877-18. 10.1128/mBio.01877-18 PubMed 30301859