pZac2.1-GfaABC1D-GAT3-HA-mCherry
(Plasmid
#117722)
-
PurposeExpresses a GABA transporter GAT-3 (Slc6a11)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepZac2.1
- Backbone size w/o insert (bp) 4454
- Total vector size (bp) 7153
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSlc6a11
-
Alt nameGABA transporter 3
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1878
-
Entrez GeneSlc6a11 (a.k.a. D930045G19Rik, E130202I16Rik, GAT4, Gabt4, Gat3)
-
Tags
/ Fusion Proteins
- HA (C terminal on insert)
- mCherry (C terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pZac2.1-GfaABC1D-GAT3-HA-mCherry was a gift from Baljit Khakh (Addgene plasmid # 117722 ; http://n2t.net/addgene:117722 ; RRID:Addgene_117722) -
For your References section:
Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal Microcircuits and Causes Repetitive Behavior. Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS. Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 10.1016/j.neuron.2018.08.015. 10.1016/j.neuron.2018.08.015 PubMed 30174118
Map uploaded by the depositor.
